abstract：:The objective of the study is to explore the possible association of the monocyte chemoattractant protein (MCP)-1-362G/C genetic polymorphism and plasma levels of MCP-1 in patients with spinal tuberculosis (TB). The MCP-1-362G/C (rs2857656) polymorphism and blood levels of MCP-1 in patients with spinal TB and healthy ...

journal_title：Diagnostic microbiology and infectious disease

authors： Guo C,Zhang H,Gao Q,He D,Tang M,Liu S,Deng A,Wang Y,Lu S,Li J,Yin X,Guo Q

更新日期：2014-01-01 00:00:00

abstract：:Antibiotic-loaded acrylic bone cements (ALABC) spacers are routinely used in the treatment of prosthetic joint infections. The objectives of our study were to evaluate different ALABC for elution kinetics, thermal stability, and mechanical properties. A 10 or 20% mixture (w/w) beads of medium viscosity bone cement (De...

journal_title：Diagnostic microbiology and infectious disease

authors： Gálvez-López R,Peña-Monje A,Antelo-Lorenzo R,Guardia-Olmedo J,Moliz J,Hernández-Quero J,Parra-Ruiz J

更新日期：2014-01-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis) is the most common causative agent of non-typhoidal salmonellosis in Malaysia. We aimed to characterize S. Enteritidis isolated from humans and animals by analyzing their antimicrobial resistance profiles and genotypes. A total of 111 strains were characterized ...

journal_title：Diagnostic microbiology and infectious disease

authors： Ngoi ST,Thong KL

更新日期：2013-12-01 00:00:00

abstract：:A total of 93 infertile and 70 fertile men attending various urology and gynecology clinics in Jordan were investigated in this prospective study. First void urine and the corresponding semen specimens were collected from 96% of the patients. Presence of Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplas...

journal_title：Diagnostic microbiology and infectious disease

authors： Abusarah EA,Awwad ZM,Charvalos E,Shehabi AA

更新日期：2013-12-01 00:00:00

abstract：:We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA su...

journal_title：Diagnostic microbiology and infectious disease

authors： Mc Gann P,Milillo M,Kwak YI,Quintero R,Waterman PE,Lesho E

更新日期：2013-11-01 00:00:00

abstract：:A patient presented with inflamed hands and Erysipelothrix rhusiopathiae bacteremia. Because a high incidence of endocarditis has been reported with this organism, a transesophageal echocardiogram was obtained, which was normal. Treatment with oral moxifloxacin resolved all manifestations of illness. The association b...

journal_title：Diagnostic microbiology and infectious disease

authors： Drekonja DM

更新日期：2013-11-01 00:00:00

abstract：:Acinetobacter baumannii has emerged as a highly problematic hospital-associated pathogen. Different mechanisms contribute to the formation of multidrug resistance in A. baumannii, including the AdeABC efflux system. Distribution of the structural and regulatory genes encoding the AdeABC efflux system among genetically...

journal_title：Diagnostic microbiology and infectious disease

authors： Wieczorek P,Sacha P,Czaban S,Hauschild T,Ojdana D,Kowalczuk O,Milewski R,Poniatowski B,Nikliński J,Tryniszewska E

更新日期：2013-10-01 00:00:00

abstract：:The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected case...

journal_title：Diagnostic microbiology and infectious disease

authors： Shinu P,Singh V,Nair A,Mehrishi P,Mehta S,Joshi E

更新日期：2013-10-01 00:00:00

abstract：:We isolated an Escherichia coli O157:H7 strain that was negative for verocytotoxin production, but positive for the vtx2 gene using commercial kits, from an asymptomatic food handler. The laboratory investigations revealed that a 1310-bp insertion sequence, IS1203 variant, was present in the B subunit-coding region of...

journal_title：Diagnostic microbiology and infectious disease

authors： Harada T,Hirai Y,Itou T,Hayashida M,Seto K,Taguchi M,Kumeda Y

更新日期：2013-10-01 00:00:00

abstract：:The application of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth has potential to identify bloodstream infection earlier and facilitate timely management. We prospectively tested a novel, rapid, and inexpensive in-house spin-lysis protocol...

journal_title：Diagnostic microbiology and infectious disease

authors： Gray TJ,Thomas L,Olma T,Iredell JR,Chen SC

更新日期：2013-10-01 00:00:00

abstract：:The purpose of this study was to explore the role of Treg cells, Th17 cells and cytokines associated with Treg/Th17 differentiation in the occurrence, development and outcome of chronic hepatitis B (CHB). To do so, we detected populations of Treg and Th17 cells and their associated cytokines in the peripheral blood of...

journal_title：Diagnostic microbiology and infectious disease

authors： Su ZJ,Yu XP,Guo RY,Ming DS,Huang LY,Su ML,Deng Y,Lin ZZ

更新日期：2013-08-01 00:00:00

abstract：:Proctitis caused by Chlamydia trachomatis L2b can manifest with very mild, nonspecific symptoms, and appropriate diagnostic evaluation is crucial. The case report demonstrates that rapid screening test, detection of specific antibodies in serum, and direct pathogen identification by PCR performed on tissue sample or r...

journal_title：Diagnostic microbiology and infectious disease

authors： Markowicz M,Grilnberger E,Huber F,Leibl G,Abrahamian H,Gartner M,Huber M,Chott A,Reiter M,Stanek G

更新日期：2013-08-01 00:00:00

abstract：:To find predictive genetic risk markers for strong biofilm producers of Staphylococcus aureus, we studied the relatedness of agr and SCCmec types and fnbB and IS256 genes to biofilm-forming ability in 465 clinical isolates. fnbB and SCCmec type III are candidates as genetic risk predictors for the strong biofilm produ...

journal_title：Diagnostic microbiology and infectious disease

authors： Lim Y,Shin HJ,Kwon AS,Reu JH,Park G,Kim J

更新日期：2013-08-01 00:00:00

abstract：:Rapid and accurate diagnostic tests for Clostridium difficile infections (CDI) are crucial for management of patients with suspected CDI and for infection control. Enzyme immunoassays for detection of the toxins are routinely used but lack adequate sensitivity. We generated slow off-rate modified aptamers (SOMAmer™ re...

journal_title：Diagnostic microbiology and infectious disease

authors： Ochsner UA,Katilius E,Janjic N

更新日期：2013-07-01 00:00:00

abstract：OBJECTIVES:Routine use of disk diffusion tests for detecting antibiotic resistance in Legionella pneumophila has not been described. The goal of this study was to determine the correlation of MIC values and inhibition zone diameter (MDcorr) in clinical L. pneumophila isolates. METHODS:Inhibition zone diameter of 183 L...

journal_title：Diagnostic microbiology and infectious disease

authors： Bruin JP,Diederen BM,Ijzerman EP,Den Boer JW,Mouton JW

更新日期：2013-07-01 00:00:00

abstract：:The objective of the study is to compare the performance of conventional fluorescence microscopy (CFM) and light-emitting diode (LED) fluorescence microscopy (FM) for detection of acid-fast bacilli (AFB) in clinical samples. We included AFB smears, stained using the auramine O method and blindly examined with both CFM...

journal_title：Diagnostic microbiology and infectious disease

authors： Marzouk M,Ferjani A,Dhaou M,Ali MH,Hannachi N,Boukadida J

更新日期：2013-07-01 00:00:00

abstract：:Biomarkers such as procalcitonin (PCT) have been studied to guide duration of antibiotic therapy. We aimed to assess whether a decrease in PCT levels could be used to reduce the duration of antibiotic therapy in intensive care unit (ICU) patients with a proven infection without risking a worse outcome. We assessed 265...

journal_title：Diagnostic microbiology and infectious disease

authors： Deliberato RO,Marra AR,Sanches PR,Martino MD,Ferreira CE,Pasternak J,Paes AT,Pinto LM,dos Santos OF,Edmond MB

更新日期：2013-07-01 00:00:00

abstract：:Haemophilus influenzae rarely causes acute endometritis and the few published cases have always been associated with intrauterine devices (IUD). A 48-year-old female presented to the emergency department with a 3-day history of lower abdominal pain and fever. On physical examination she was tachycardic, hypotensive an...

journal_title：Diagnostic microbiology and infectious disease

authors： Martin D,Dbouk RH,Deleon-Carnes M,del Rio C,Guarner J

更新日期：2013-06-01 00:00:00

abstract：:To support antimicrobial stewardship, some healthcare systems have begun creating outpatient antibiograms. We developed inpatient and primary care outpatient antibiograms for a regional health maintenance organization (HMO) and academic healthcare system (AHS). Antimicrobial susceptibilities from 16,428 Enterococcus, ...

journal_title：Diagnostic microbiology and infectious disease

authors： McGregor JC,Bearden DT,Townes JM,Sharp SE,Gorman PN,Elman MR,Mori M,Smith DH

更新日期：2013-05-01 00:00:00

abstract：:An ongoing program of international generic antimicrobial potency assays for piperacillin/tazobactam has been summarized here through December 2010, and the initial results for meropenem generic lots from the United States are also presented. Fifteen additional piperacillin/tazobactam generic lots revealed an average ...

journal_title：Diagnostic microbiology and infectious disease

authors： Jones RN,Sader HS,Flamm RK,Watters AA

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:We evaluated the clinical significance of urine cultures from patients with an indwelling urinary catheter (UC) from which 2 different pathogens were isolated. METHODS:Urine cultures from patients with a UC from which 2 different organisms were isolated were randomly divided into a control group (culture re...

journal_title：Diagnostic microbiology and infectious disease

authors： Wiener-Well Y,Gofman I,Assous MV,Freier-Dror Y,Yinnon AM,Lachish T

更新日期：2013-05-01 00:00:00

abstract：:The clinical features of abdominal tuberculosis (TB) are non-specific and establishing a diagnosis remains a challenge. A delay in diagnosis is likely to increase the morbidity in these patients. We developed a multiplex polymerase chain reaction (PCR) using 16SrRNA, IS6110, and devR, and evaluated it in comparison wi...

journal_title：Diagnostic microbiology and infectious disease

authors： Hallur V,Sharma M,Sethi S,Sharma K,Mewara A,Dhatwalia S,Yadav R,Bhasin D,Sinha SK,Rana S,Joshi K

更新日期：2013-05-01 00:00:00

abstract：:GSK2251052 is a boron-containing antimicrobial agent in clinical development for the treatment of serious Gram-negative bacterial infections. These GSK2251052 quality control (QC) studies were performed to establish ranges for control strains (broth microdilution [BMD] MIC and disk diffusion zones) as follows: Pseudom...

journal_title：Diagnostic microbiology and infectious disease

authors： Ross JE,Scangarella-Oman N,Jones RN

更新日期：2013-04-01 00:00:00

abstract：:The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombin...

journal_title：Diagnostic microbiology and infectious disease

authors： Pierimarchi P,Cerni L,Alarcón de Noya B,Nicotera G,Díaz-Bello Z,Angheben A,Scacciatelli D,Zonfrillo M,Recinelli G,Serafino A

更新日期：2013-04-01 00:00:00

abstract：:Clostridium difficile infections (CDI) is a leading cause of nosocomial infections worldwide. The changes in the epidemiology of CDI during the past years, including the appearance of new epidemic strains of C. difficile that cause CDI episodes with increased severity, have led to the development of molecular methods ...

journal_title：Diagnostic microbiology and infectious disease

authors： Hernández-Rocha C,Barra-Carrasco J,Álvarez-Lobos M,Paredes-Sabja D,Guzmán-Durán AM

更新日期：2013-04-01 00:00:00

abstract：:Fluorescent and colorimetric reporter genes are valuable tools for drug screening models, since microscopy is labor intensive and subject to observer variation. In this work, we propose a fluorimetric method for drug screening using red fluorescent parasites. Fluorescent Leishmania amazonensis were developed after tra...

journal_title：Diagnostic microbiology and infectious disease

authors： Rocha MN,Corrêa CM,Melo MN,Beverley SM,Martins-Filho OA,Madureira AP,Soares RP

更新日期：2013-03-01 00:00:00

abstract：:Ceftaroline fosamil resulted in higher cure rates than ceftriaxone in patients with community-acquired bacterial pneumonia in 2 randomized trials (FOCUS 1 and FOCUS 2). The present analysis examines the subgroup of patients with Streptococcus pneumoniae infection to determine whether the apparent difference in cure ra...

journal_title：Diagnostic microbiology and infectious disease

authors： Shorr AF,Kollef M,Eckburg PB,Llorens L,Friedland HD

更新日期：2013-03-01 00:00:00

abstract：:Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cyto...

journal_title：Diagnostic microbiology and infectious disease

authors： Nikolova M,Markova R,Drenska R,Muhtarova M,Todorova Y,Dimitrov V,Taskov H,Saltini C,Amicosante M

更新日期：2013-03-01 00:00:00

abstract：:Microarrays are widely used in high-throughput DNA and RNA hybridization tests and recently adopted to protein and small molecule interaction studies in basic research and diagnostics. Parallel detection of serum antibodies and antigens has several potential applications in epidemiologic research, vaccine development,...

journal_title：Diagnostic microbiology and infectious disease

authors： Viitala SM,Jääskeläinen AJ,Kelo E,Sirola H,Moilanen K,Suni J,Vaheri A,Vapalahti O,Närvänen A

更新日期：2013-02-01 00:00:00

abstract：:Melioidosis is caused by the Gram-negative bacterium Burkholderia pseudomallei. The gold standard for diagnosis is culture, which requires at least 3-4 days to obtain a result, hindering successful treatment of acute disease. An indirect haemagglutination assay (IHA) is often used but lacks sensitivity. Approximately ...

journal_title：Diagnostic microbiology and infectious disease

authors： Cooper A,Williams NL,Morris JL,Norton RE,Ketheesan N,Schaeffer PM

更新日期：2013-02-01 00:00:00

abstract：:The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ-producing cell...

journal_title：Diagnostic microbiology and infectious disease

authors： Kim S,Kim YK,Lee H,Cho JE,Kim HY,Uh Y,Kim YM,Kim H,Cho SN,Jeon BY,Lee H

更新日期：2013-01-01 00:00:00

abstract：:A 14-year longitudinal survey of Streptococcus pneumoniae (18,911) strains by the SENTRY Antimicrobial Surveillance Program shows significant increases in resistance to amoxicillin/clavulanate (18.9%), penicillin at a MIC of ≥ 4 μg/mL (14.8%), and ceftriaxone (11.7%) among 2011 isolates. Fluoroquinolones (levofloxacin...

journal_title：Diagnostic microbiology and infectious disease

authors： Jones RN,Sader HS,Mendes RE,Flamm RK

更新日期：2013-01-01 00:00:00

abstract：:The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was perform...

journal_title：Diagnostic microbiology and infectious disease

authors： Herremans T,Hogema BM,Nabuurs M,Peeters M,Wegdam-Blans M,Schneeberger P,Nijhuis C,Notermans DW,Galama J,Horrevorts A,van Loo IH,Vlaminckx B,Zaaijer HL,Koopmans MP,Berkhout H,Socolovschi C,Raoult D,Stenos J,Nicholson W

更新日期：2013-01-01 00:00:00

abstract：:Since the introduction of the Haemophilus influenzae serotype b vaccine, invasive H. influenzae disease has become dominated by nontypeable (NT) strains. Several widely used molecular diagnostic methods have been shown to lack sensitivity or specificity in the detection of some of these strains. Novel real-time assays...

journal_title：Diagnostic microbiology and infectious disease

authors： Meyler KL,Meehan M,Bennett D,Cunney R,Cafferkey M

更新日期：2012-12-01 00:00:00

abstract：:We determined the frequency of isolation of non-aureus staphylococcal small colony variants (SCVs) from 31 patients with staphylococcal prosthetic joint infection (PJI) and described the antimicrobial susceptibility, auxotrophy, and biofilm-forming capacity of these SCVs. Eleven non-aureus SCVs were recovered, all of ...

journal_title：Diagnostic microbiology and infectious disease

authors： Maduka-Ezeh AN,Greenwood-Quaintance KE,Karau MJ,Berbari EF,Osmon DR,Hanssen AD,Steckelberg JM,Patel R

更新日期：2012-11-01 00:00:00

abstract：:Three persons became victims of a serial knife stabbing in a fight. Three months later, one of the victims presented with acute hepatitis. On the fifth day of his admission to the hospital, another victim of this serial stabbing presented to our clinic with acute hepatitis B infection. The patients told that the first...

journal_title：Diagnostic microbiology and infectious disease

authors： Bodur H,Onguru P,Akıncı E,Kanyılmaz D,Yetkin A,Ozdemir B,But A,Eren S

更新日期：2012-11-01 00:00:00

abstract：:Acinetobacter species isolates from a range of environments, including soil, were investigated. We determined 16S rRNA and rpoB gene sequences for species identification and performed tests of antimicrobial resistance susceptibility. Twenty-nine of the isolates (8 from soil and 21 from life environment) belonged to th...

journal_title：Diagnostic microbiology and infectious disease

authors： Choi JY,Kim Y,Ko EA,Park YK,Jheong WH,Ko G,Ko KS

更新日期：2012-10-01 00:00:00

abstract：:Recent reports suggest that false-positive rifampicin resistance may be assigned by the Xpert MTB/RIF assay. We analysed 169 specimens using the MTB/RIF assay. Using culture as the gold standard, we found that the assay had 100% sensitivity and specificity for detecting M. tuberculosis. However, we found that the assa...

journal_title：Diagnostic microbiology and infectious disease

authors： Williamson DA,Basu I,Bower J,Freeman JT,Henderson G,Roberts SA

更新日期：2012-10-01 00:00:00

abstract：:We analyse the diversity of extended-spectrum beta-lactamases (ESBLs), antibiotic resistance genes, and plasmid content among Escherichia coli from a community and a hospital setting in Rio de Janeiro, Brazil. ESBL producers (CTX-M-2, CTX-M-9, CTX-M-59) belonged to different phylogroups/sequence types, and bla(CTX-M) ...

journal_title：Diagnostic microbiology and infectious disease

authors： Queiroz ML,Antunes P,Mourão J,Merquior VL,Machado E,Peixe LV

更新日期：2012-09-01 00:00:00

abstract：:Combining a polymerase chain reaction (PCR) test with bronchoscopy is frequently performed to allow a rapid diagnosis of smear-negative pulmonary tuberculosis (PTB). However, limited data are available concerning clinical judgment in patients with suspected PTB and AFB smear- and PCR-negative bronchial aspirates (BA)....

journal_title：Diagnostic microbiology and infectious disease

authors： Lee J,Lee SY,Yoo SS,Cha SI,Won DI,Park JY,Lee WK,Kim CH

更新日期：2012-07-01 00:00:00